CBSE Class 12 Biotechnology Syllabus for Board Exam 2026-27, Download PDF Here
The CBSE Class 12 Biotechnology syllabus for the 2026-27 academic year remains same as the previous year. It outlines the required chapters and subjects, assessment criteria, project work guidelines, and the overall course structure for students preparing for the board exams.
The Central Board of Secondary Education has published the Class 12 syllabus for the 2026-27 year. You can find this syllabus on the Central Board of Secondary Education Academic website. The Class 12 syllabus is very important for students and teachers. It is a guide that tells you what subjects and chapters you will be covering and weightage it is carrying. You can download the CBSE Class 12th Biotechnology Syllabus from the link mentioned here.
It tells you what the course is about and how it is organized. The Class 12 syllabus also tells you how much each chapter is worth for the 2027 board examinations. If you get the Class 12 syllabus at the start of the year you can prepare for the exams well. The Central Board of Secondary Education Class 12 Biotechnology syllabus, for 2025-26 is available to download.
CBSE Class 12 Biotechnology Syllabus: COURSE STRUCTURE
The complete course structure give the details about the unit-wise distribution of marks for both theoretical concepts and practical assessments. Designed to be completed within a 3-hour duration, the curriculum allocates a maximum of 100 marks divided into a 70-mark theory component and a 30-mark practical examination. This structured approach ensures a balanced evaluation of a student's core conceptual understanding and hands-on laboratory skills.
Detailed Course Structure Breakdown
| Units | Subject / Content Topic | Marks Allocated |
| Unit V | Protein and Gene Manipulation | 40 |
| Unit VI | Cell Culture and Genetic Manipulation | 30 |
| Practicals | 30 | |
| Total | 100 |
Unit-V Protein and Gene Manipulation 40 Marks
Chapter-1: Recombinant DNA Technology
Introduction, Tool of Recombinant DNA technology, making rDNA molecule, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.
Chapter-2: Protein Structure and Engineering
Introduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)
Chapter-3: Genomics, Proteomics and Bioinformatics
Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, Analysis using bioinformatics tools.
Unit-VI Cell Culture and Genetic Manipulation 30 Marks
Chapter-1: Microbial Cell Culture and its Applications
Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.
Chapter -2: Plant Cell Culture and Applications
Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Transgenic plants with beneficial traits, Biosafety of transgenic plants
Chapter-3: Animal Cell Culture and Applications
Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.
PRACTICALS 30 Marks
Note: Every student will be required to do the following experiments during the academic session.
1. Use of special equipment in biotechnology experiments
2. Isolation of bacterial plasmid DNA
3. Detection of DNA by gel electrophoresis
4. Estimation of DNA by UV spectroscopy
5. Isolation of bacteria from curd & staining of bacteria
6. Cell viability assay using Evan’s blue dye exclusion method
7. Data retrieval and database search using internet site NCBI and download a DNA and protein sequence from internet, analyze it and comment on it
8. Reading of a DNA sequencing gel to arrive at the sequence
9. Project work
Prescribed Books:
1. A Text Book of Biotechnology - Class XI : Published by CBSE, New Delhi
2. As reference- Biotechnology - Class XI : Published by NCERT, New Delhi
3. A Laboratory Manual of Biotechnology - Class XI : Published by CBSE, New Delhi
4. A Text Book of Biotechnology - Class XII : Published by CBSE, New Delhi
5. A Laboratory Manual of Biotechnology - Class XII : Published by CBSE, New
Delhi
To downoad complete Biotechnology class 12 syllabus for the academic yearf 2025-26. Studnts can click on the below mentioned link and download the full PDF for free.
CBSE Class 12 Biotechnology Syllabus 2026-27 Downlaod PDF |
The CBSE Class 12 Biotechnology Syllabus provides a clear roadmap for students preparing for their 2027 board exams. The detailed syllabus helps in understanding the course structure, subject-wise weightage, and chapter-wise breakdown, facilitating a well-organized study plan and successful academic outcomes.
Anisha Mishra is journalist with over 3 years of experience in covering the Indian education sector. She has worked extensively in the K12 domain, with focus on the state board as well as central board examinations, policy structure of seconday and higher secondary education as well as the entrance examinations like JEE, NEET, CLAT, etc. Her extensive experience in the domain has helped her provide students with concise accurate information in all aspectes of school life and education. Her key interest lies in decoding the changes in the curriculum, NEP implementation and changing education ecosystem in the country. Besides working, she enjoys traveling, exploring new places and cultures, and painting.
